Did you know about our mouse linkage marker set?
28 July 2013Mouse Linkage Marker Set
The mouse linkage Market set (MLMS) is a panel that allows you to perform linkage mapping studies. The panel is run using our NATA accredited Agena SNP genotyping service.
The panel contains over 550 SNPs with an average distance of 3-5cM across the mouse genome and provides approximately 300 informative markers between common mouse crosses. The SNPs have been carefully selected to maximise the polymorphism between common inbred strains.
Applications
Speed Congenics
What is speed congenics?
A congenic strain is identical to a reference strain at all genetic loci except one. The differing locus is usually the transgene or knockout region of interest. Congenic strains are widely used in biomedical research because they reduce genetic variability and provide insight into the contribution of genetic background to phenotype.
Benefit of using Mouse Linkage Marker Sets
The MLMS can be used to preferentially select animal carrying the locus of interest for further breeding by identifying the highest percentage of recipient versus donor strain DNA. This accelerates the speed of congenic strain production. Utilizing traditional, random backcrossing methods, it would take 10 generations (upwards of 2.5 years) to produce a comparable congenic strain.
Mouse strain validation
The panel can be used to validate in house mouse strains, ensuring consistency with established reference strains.
Technical Details
|
Submission Format |
DNA requirements |
Number of Informative SNPs between Common Mouse Strains
| sv | aj | akr | balb | btbr | c3h | b6 | dba | fvb | nod | nzb | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| aj | 430 | ||||||||||
| akr | 363 | 308 | |||||||||
| balb | 377 | 245 | 322 | ||||||||
| btbr | 248 | 397 | 350 | 355 | |||||||
| c3h | 403 | 296 | 341 | 297 | 390 | ||||||
| b6 | 400 | 451 | 361 | 396 | 350 | 400 | |||||
| dba | 407 | 405 | 382 | 389 | 370 | 269 | 392 | ||||
| fvb | 332 | 346 | 364 | 344 | 322 | 361 | 352 | 359 | |||
| nod | 356 | 345 | 333 | 356 | 347 | 362 | 350 | 357 | 274 | ||
| nzb | 328 | 398 | 348 | 380 | 327 | 389 | 345 | 330 | 340 | 338 | |
| sjl | 341 | 343 | 332 | 328 | 340 | 350 | 359 | 356 | 232 | 316 |
Common Strains Covered by Panel
| 129S4/SvJae | C57BR/cdj | FVB/NJ | NOD/ShiLtJ | SEA/GnJ |
|---|---|---|---|---|
| 129X1/SvJ | C57L/J | I/LnJ | NON/ShiLtJ | SJL/J |
| A/J | C58/J | JF1/Ms | NZB/BINJ | SM/J |
| AKR/J | CAST/EiJ | KK/HIJ | NZW/LacJ | SPRET/EiJ |
| BALB/cByJ | CBA/J | LG/J | O20 | ST/bJ |
| BTBR_T+tf/J | CE/J | LP/J | PERA/EiJ | SWR/J |
| BUB/BnJ | CZECHII/EiJ | MA/MyJ | PL/J | WSB/EiJ |
| C3H/HeJ | DBA/1J | MAI/Pas | PWD/PhJ | |
| C57BL/6J | DBA/2J | MOLF/EiJ | Qsi5 |
Results format and delivery
Results are reported as an Excel spread sheet of genotypes plus we provide a % similarity to the backcross strain.
Also Available
DNA Extraction service
Don’t want to extract your mouse DNA? We can do it for you with our DNA extraction service. Learn more ->
Fine Mapping services
Combine with our fine mapping service to map targeted regions to the mouse genome. Learn more ->