The fragment analysis service offered by the AGRF provides both capillary separation and analysis services for fluorescently labelled DNA fragments. Capillary electrophoresis is used for fragment separation and data is analysed using AB GeneMapper software. Marker types applied to the service include microsatellites, AFLP (amplified fragment length polymorphisms), T-RFLP (terminal restriction fragment length polymorphisms) and MLPA (multiplex ligation-dependent probe amplification), for which analysis can be customised to your marker type and project requirements.
For this service the customer performs PCR and provides the AGRF with PCR product for electrophoresis. The DNA Analyser platform can accommodate up to four different coloured fluorescent dyes: 6-FAM, VIC, NED or PET. This allows multiple markers to be amplified (for the same individual), pooled and then run in a single capillary. Where multiple markers of the same colour are pooled, it is important to ensure that the fragment size range of markers does not overlap. A maximum fragment length of 1,000 base pairs can be analysed.